解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
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公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
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外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.06.006
更新日期:2005-10-01 00:00:00
abstract::Despite some 26 published reports addressing oyster sperm cryopreservation, systematic factor optimization is lacking, and sperm cryopreservation has not yet found application in aquaculture on a commercial scale. In this study, the effects of cooling rate, single or combined cryoprotectants at various concentrations,...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.06.007
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:The effect of dimethyl sulfoxide (Me2SO) on enumeration of post-thaw CD45+ and CD34+ cells of umbilical cord blood (HPC-C) and mobilized peripheral blood (HPC-A) has not been systematically studied. METHODS:Cells from leukapheresis products from multiple myeloma patients and umbilical cord blood cells were ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.06.003
更新日期:2005-10-01 00:00:00
abstract::Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.02.007
更新日期:2005-06-01 00:00:00
abstract::Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.12.004
更新日期:2005-04-01 00:00:00
abstract::Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.10.004
更新日期:2005-02-01 00:00:00
abstract::An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.08.003
更新日期:2004-12-01 00:00:00
abstract::We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.06.004
更新日期:2004-10-01 00:00:00
abstract::Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.01.010
更新日期:2004-06-01 00:00:00
abstract::Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2003.10.008
更新日期:2004-04-01 00:00:00
abstract::Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2003.12.001
更新日期:2004-02-01 00:00:00
abstract::Cold preservation and reperfusion of liver during transplantation are necessary steps in the procedure but which are also associated with damage to the organ. One aspect of this damage is thought to concern up-regulation of inflammatory markers, such as the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2003.09.003
更新日期:2003-10-01 00:00:00
abstract::Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(03)00066-x
更新日期:2003-08-01 00:00:00
abstract::The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(03)00045-2
更新日期:2003-06-01 00:00:00
abstract::Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(03)00017-8
更新日期:2003-04-01 00:00:00
abstract::Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00181-5
更新日期:2003-02-01 00:00:00
abstract::We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00123-2
更新日期:2002-10-01 00:00:00
abstract::In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00106-2
更新日期:2002-08-01 00:00:00
abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00031-7
更新日期:2002-06-01 00:00:00
abstract::Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00017-2
更新日期:2002-04-01 00:00:00
abstract::The objective of this study was to determine osmotic tolerance of canine spermatozoa. The study comprised three experiments: (1) spermatozoa suspended either in an egg yolk-citrate (EYC) extender or in Kenney skim milk extender were exposed to NaCl solutions ranging from 290 to 1500 mOsm; (2) spermatozoa suspended in ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/S0011-2240(02)00009-3
更新日期:2002-02-01 00:00:00
abstract::The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2351
更新日期:2001-11-01 00:00:00
abstract::Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...
journal_title:Cryobiology
pub_type: 杂志文章,评审
doi:10.1006/cryo.2001.2359
更新日期:2001-09-01 00:00:00
abstract::Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2339
更新日期:2001-08-01 00:00:00
abstract::The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2324
更新日期:2001-05-01 00:00:00
abstract::Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2306
更新日期:2001-03-01 00:00:00
abstract::This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2305
更新日期:2001-02-01 00:00:00
abstract::Morulae and unhatched blastocysts from Large White hyperprolific (LWh) and Meishan (MS) gilts were selected to test an ultrarapid open pulled straw (OPS) vitrification method with two media. The viability of vitrified/warmed embryos was estimated by the percentage of embryos that developed to the hatched blastocyst st...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2273
更新日期:2000-09-01 00:00:00
abstract::Temperature may have significant influence on vascular tone in such cases as organ preservation, coronary bypass surgery, and extracorporeal circulation. The aim of this research was to study the direct effect of temperature variation on vascular tone in an attempt to elucidate the mechanisms involved. In a first seri...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2263
更新日期:2000-08-01 00:00:00
abstract::The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2247
更新日期:2000-06-01 00:00:00
abstract::Uncontrolled hemorrhage is the primary cause of death in both blunt and penetrating liver trauma. Cryohemostasis was attempted in the past for elective liver surgery but did not gain popularity. During past decades, cryoequipment was refined and successfully used for tumor ablation. The purpose of the present study wa...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2239
更新日期:2000-05-01 00:00:00
abstract::Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2227
更新日期:2000-03-01 00:00:00
abstract::The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2221
更新日期:2000-02-01 00:00:00
abstract::In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2209
更新日期:1999-11-01 00:00:00
abstract::Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2199
更新日期:1999-09-01 00:00:00
abstract::We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2182
更新日期:1999-08-01 00:00:00
abstract::In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2163
更新日期:1999-06-01 00:00:00
abstract:UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2181
更新日期:1999-06-01 00:00:00
abstract::Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2146
更新日期:1999-05-01 00:00:00
abstract::A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facil...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2155
更新日期:1999-03-01 00:00:00